In-silico Dyeing strategy: Unveiling the Binding of Cotton Protein Annexin to Phyto-Pigments through Bioinformatics

Document Type : Original Article

Authors

Department of Biotechnology, Bapuji Institute of Engineering and Technology, Davangere, P.O. Box: 577004, Karnataka, India

Abstract

Natural dyes derived from plant sources such as fruits and flowers offer enhanced biodegradability and environmental compatibility compared to synthetic alternatives. This bioinformatics study explores the in-silico interactions of phyto-pigments with cotton-specific proteins, emphasizing their potential applications in the cotton textile industry. Utilizing an integrated bioinformatics approach including molecular docking, virtual screening, and visualization tools such as DS Visualizer, we investigated the binding interactions between phyto-pigments and Annexin, a key cotton protein. A total of seven phyto-pigments were examined: Malvidin, Peonidin, Cyanidin, Petunidin, Pelargonidin, Betanin, and Betacyanin. Among these, Betanin and Betacyanin found in beetroot and dragon fruit, respectively exhibited the strongest binding affinities with Annexin. These preliminary findings from in-silico studies suggest a promising role for these bio-pigments in the development of natural dyes with functional biological activity. The elucidation of specific binding modes and structural compatibilities reinforces the value of in-silico studies in advancing the application of bio-based dyes for sustainable textile dyeing processes. The study is conducted using bioinformatics tools and is focused solely on molecular docking simulations. No experimental dyeing or laboratory-based validation was undertaken at this stage. This bioinformatics study underscores the potential of phyto-pigments not only as eco-friendly colorants but also as agents capable of forming meaningful biochemical interactions within textile fibres. 

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References

  1. Wen X, Chen Z, Yang Z, Wang M, Jin S, Wang G, Zhang L, Wang L, Li J, Saeed S, He S, Wang Z, Wang K, Kong Z, Li F, Zhang X, Chen X, Zhu Y. A comprehensive overview of cotton genomics, biotechnology and molecular biological studies. Sci China Life Sci. 2023; 66(10):2214-2256. https://doi. org/10.1007/s11427-022-2278-0.
  2. Lee JJ, Woodward AW, Chen ZJ. Gene expression changes and early events in cotton fibre development. Ann Bot. 2007;100(7):1391-1401. https://doi.org/10. 1093/aob/mcm232.
  3. Basra AS, Malik C. Development of the cotton fibre. Int Rev Cytol. 1984; 89:65-113. https://doi.org/10. 1016/S0074-7696(08)61300-5.
  4. Jan M, Liu Z, Guo C, Sun X. Molecular regulation of cotton fiber development: a review. Int J Mol Sci. 2022; 23(9):5004. https://doi.org/10.3390/ijms23095 004.
  5. Kim HJ, Triplett BA. Cotton fibre growth in planta and in vitro: models for plant cell elongation and cell wall biogenesis. Plant Physiol. 2001;127:1361-1366. https://doi.org/10.1104/pp.010724.
  6. Wu X, Wang Y, Bian Y, Ren Y, Xu X, Zhou F, Ding H. A critical review on plant annexin: structure, func-tion, and mechanism. Plant Physiol Biochem. 2022; 190:81-89. https://doi.org/10.1016/j.plaphy.2022.08. 019.
  7. Luo J, Li M, Ju J, Hai H, Wei W, Ling P, Li D, Su J, Zhang X, Wang C. Genome-wide identification of the GhANN gene family and functional validation of GhANN11 and GhANN4 under abiotic stress. Int J Mol Sci. 2024;25(3):1877. https://doi.org/10.3390/ ijms25031877.
  8. Babu RS, Raghavendra G. Natural dyes: sources, chemistry, and applications. Dyes Pigments. 2014; 103: 1-10. https://doi.org/10.1016/j.dyepig.2014.01. 014.
  9. Islam T, Repon MR, Islam T, Sarwar Z, Rahman MM. Impact of textile dyes on health and ecosystem: a review of structure, causes, and potential solutions. Environ Sci Pollut Res Int. 2023; 30(4):9207-9242. https://doi.org/10.1007/s11356-022-24398-3.
  10. Tiwari BR, Kumar S. Health hazards associated with textile dyes. J Environ Health Sci Eng. 2015;13:1-8. https://doi.org/10.1186/s40201-015-0131-0.
  11. Khan S, Malik A. Environmental and health effects of textile industry wastewater. In: Malik A, Grohmann E, Akhtar R, editors. Environ Deter Human Health. Dordrecht: Springer; 2014. https://doi.org/10.1007/ 978- 94-007-7890-0_4.
  12. Repon MR, Islam T, Paul TK, et al. Natural dyes in textile printing: parameters, methods, and performance. Environ Sci Pollut Res Int. 2024; 31(35):47552-47583. https://doi.org/10.1007/s11356-024-34424-1.
  13. Repon M, Islam T, Islam T, Ghorab A, Rahman M. Cleaner pathway for developing bioactive textile materials using natural dyes: a review. Environ Sci Pollut Res Int. 2023; 30:48793-48823. doi:10.1007/ s11356-023-26131-0.
  14. Lyu X, Lyu Y, Yu H, Chen W, Ye L, Yang R. Biotechnological advances for improving natural pigment production: a state-of-the-art review. Bio-resour Bioprocess. 2022; 9:497-504. https://doi.org/ 10.1186/s40643-022-00497-4.
  15. Gupta VK. Fundamentals of Natural Dyes and Its Application on Textile Substrates. London: Intech Open; 2019. https://doi.org/10.5772/intechopen.899 64.
  16. Che J, Yang X. A recent (2009–2021) perspective on sustainable color and textile coloration using natural plant resources. Heliyon. 2022; 8:e10979. https://doi. org/10.1016/j.heliyon.2022.e10979.
  17. Chungkrang L, Bhuyan S. Natural dye sources and its applications in textiles: a brief review. Int J Curr Microbiol App Sci. 2020; 9(10):261-269. https://doi.org/10.20546/ijcmas.2020.910.034.
  18. Khoo HE, Azlan A, Tang ST, Lim SM. Antho-cyanidins and anthocyanins: colored pigments as food, pharmaceutical ingredients, and the potential health benefits. Food Nutr Res. 2017; 61(1):1361779. https://doi.org/10.1080/16546628.2017.1361779.
  19. He J, Giusti MM. Anthocyanins: natural colorants with health-promoting properties. Annu Rev Food Sci Technol. 2010;1:163-187. https://doi.org/10.1146/ annurev.food.080708.100754.
  20. Rodríguez-Mena A, Ochoa-Martínez LA, González-Herrera SM, Rutiaga-Quiñones OM, González-Laredo RF, Olmedilla-Alonso B. Natural pigments of plant origin: classification, extraction and application in foods. Food Chem. 2023; 398:133908. https://doi. org/10.1016/j.foodchem.2022.133908.
  21. He F, Mu L, Yan GL, et al. Biosynthesis of anthocyanins and their regulation in colored grapes. Food Chem. 2010; 119(1):6-13. https://doi.org/10. 1016/j.foodchem.2009.06.055.
  22. Brouillard R. The structure and stability of anthocyanins. Adv Food Res. 1982; 28:1-40.
  23. Vankar PS, Shukla D. Natural dyeing with antho-cyanins from Hibiscus rosa-sinensis flowers. J Appl Polym Sci. 2011;122:3361-3368. doi:10.1002/app. 34415.
  24. Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. London: Chapman & Hall; 1998.
  25. Khan B, Sindhyan R, Divan A, Rathod S. Extraction, characterization and applications of natural dyes. Ann Plant Sci. 2021;7(11):2463. https://doi.org/10. 21746/ aps.2018.7.11.4.
  26. Nayak CA. Forward osmosis membrane concen-tration process of anthocyanins from pomegranate fruit. Res Rev J Biotechnol. 2014; 4(1):2231-826.
  27. Lutamyo N, Liberato H. Extraction of natural dyes from Delonix regia flowers for cotton fibres. Tanzania J Sci. 2024; 50(1):126-133. https://doi.org/10.4314/ tjs.v50i1.10.
  28. Sakshi AK, Vaishnavi SK, Ravindra LB, Pooja RH. Hibiscus sabdariffa: a comprehensive review of its phytochemistry, traditional uses and therapeutic potentials. Int J Herbal Med. 2025; 13(4):37-41. https://doi.org/10.22271/flora.2025.v13.i4a.996.
  29. Gandía-Herrero F, Escribano J, García-Carmona F. Betaxanthins as pigments responsible for visible fluorescence in flowers. J Nat Prod. 2005; 68(3):437-439. https://doi.org/10.1021/np049699z.
  30. Esatbeyoglu T, Wagner AE, Schini-Kerth VB, Rimbach G. Betanin-a food colorant with biological activity. Mol Nutr Food Res. 2015; 59(1):36-47. https://doi.org/10.1002/mnfr.201400484.
  31. Carreón-Hidalgo JP, Franco-Vásquez DC, Gómez-Linton DR, Pérez-Flores LJ. Betalain plant sources, biosynthesis, extraction, stability enhancement methods, bioactivity, and applications. Food Res Int. 2022; 151:110821. https://doi.org/10.1016/j.foodres. 2021.110821.
  32. Alves MBN, Jorge AMS, Pereira JFB. The biotechnology revolution in textile dyeing. Trends Biotechnol. 2024;42(10):1211-1214. https://doi.org/ 10.1016/j.tibtech.2024.02.010.
  33. Repon MR, Islam T, Paul TK, Jurkonienė S, Haji A, Shukhratov S, Toki GFI. Natural dyes in textile printing: parameters, methods, and performance. Environ Sci Pollut Res Int. 2024; 31(35):47552-47583. https://doi.org/10.1007/s11356-024-34424-1.
  34. Strack D, Vogt T, Schliemann W. Recent advances in betalain research. Phytochemistry. 2003;62(3):247-269. https://doi.org/10.1016/S0031-9422(02)00664-4. 

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