Amylase Production Optimization and Textile Application of a Novel Bacillus Halotolerans SH1 Isolate from Textile Wastewater

Articles in Press

Document Type : Original Article

Authors

1 Department of Textile Engineering, Yazd University, P. O. Box: 89195-741, Yazd, Iran

2 Textile department, Yazd university

3 Department of Biology, Yazd University, P. O. Box: 89195-741, Yazd, Iran

Abstract

Textile wastewater usually contains toxic compound and a few bacteria survive in this condition. Microorganisms in textile wastewater can produce a variety of enzymes and biotechnology products. This study aimed to isolate bacteria that produce amylase and polyhydroxyalkanoates (PHAs) from textile waste. Both of these compounds are used in the textile industry. Amylase enzyme is used to enhance thread strength and PHAs for the packaging and production of suture thread, surgical meshes, cardiovascular fabrics, and conventional fibers. The highest enzyme activity was optimized using the response surface method (RSM). The isolate was identified as Bacillus halotolerans SH1 by determining the 16S rRNA gene sequence. Optimum conditions for maximum enzyme production (0.26 U/mg) were starch 5.5 g/L, temperature 37°C, and incubation period 48 hours. The results showed that a PHA concentration of 0.27 g/L is obtained from this bacterium. The reduction in BOD, COD by bacteria was found to be 54.93% and 51.92%, respectively. This study reports a new strain of Bacillus halotolerans SH1, isolated from toxic textile wastewater, which is capable of producing both PHA and α-amylase enzyme, while also significantly reducing BOD and COD levels, showing its multifunctional potential in environmental and industrial applications, especially in the textile industry.

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Main Subjects

Progress in Color, Colorants and Coatings

Articles in Press, Accepted Manuscript
Available Online from 28 July 2025

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