Cochineal Carmine Adsorbed on Layered Zinc Hydroxide Salt: Responsible for the Reddish-Pink Color of Cooked Hams Without Adding Curing Salts

Document Type : Original Article

Authors

1 Departamento de Alimentos, Programa de Pós-graduação em Tecnologia de Alimentos,‏ Universidade Tecnológica Federal do Paraná, P.O. Box: 85884-000, Medianeira‎‏, Brasil

2 Departamento de Química, Universidade Tecnológica Federal do Paraná, P.O. Box: 85884-000, Medianeira‎‏, Brasil

3 Departamento de Física, Universidade Tecnológica Federal do Paraná, P.O. Box: 85884-000, Medianeira, Brasil

Abstract

Color is one of the main attributes used to select or reject meat products, with the characteristic reddish-pink color of cured meats being developed by adding nitrate salts, which are rejected by many consumers due to their harmful effects on health. This study aimed to apply a hybrid dye (ZHN-carmine) synthesized from layered zinc hydroxide salt (ZHN) to produce a reddish-pink color in sliced cooked ham and to evaluate color stability during storage. The ham samples were prepared with cochineal carmine and hybrid dye (ZHN-carmine), sliced, vacuum-packed, and non-vacuum-packed, and exposed to white fluorescent light (1100 LUX) (5 ± 1 °C). The instrumental color measurement was performed at 0, 1, 4, 6, 8, 11, 13, and 15 day intervals. Distinction between the vacuum-packed ham samples and the non-vacuum-packed ham samples was possible based on a* value (red color intensity), which showed the importance of oxygen removal for red color stability. The reddish-pink color was more intense in the ham added with ZNH-carmine, and no color reduction was observed over the days, irrespective of it being vacuum-packed or not. Cochineal carmine adsorbed onto layered zinc hydroxide salt may be a potential replacement for curing salts regarding color formation in vacuum-packed or non-vacuum-packed cooked ham.

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References

  1. USDA. Livestock and poultry: world markets and trade. United States Department of Agriculture and Foreign Agricultural Service. 2024. p. 1–16. Available from: https://apps.fas.usda.gov/psdonline/circulars/ livestock_poultry.pdf.
  2. OECD-FAO AO. Meat consumption. OECD. 2023. Available from: https://data.oecd.org/agroutput/meat-consumption.htm.
  3. Baldin JC, Michelin EC, Polizer YJ, Rodrigues I, de Godoy SHS, Fregonesi RP, et al. Microencapsulated jabuticaba (Myrciaria cauliflora) extract added to fresh sausage as natural dye with antioxidant and antimicrobial activity. Meat Sci. 2016; 118: 15-21. https://doi.org/10.1016/j.meatsci.2016.03.016.
  4. Bohrer BM. An investigation of the formulation and nutritional composition of modern meat analogue products. Food Sci Hum Wellness. 2019; 8(4): 320-329. https://doi.org/10.1016/j.fshw.2019.11.006.
  5. Dey S, Nagababu BH. Applications of food color and bio-preservatives in the food and its effect on the human health. Food Chem Adv. 2022; 1: 100019. https://doi.org/10.1016/j.focha.2022.100019.
  6. Martins N, Roriz CL, Morales P, Barros L, Ferreira ICFR. Food colorants: Challenges, opportunities and current desires of agro-industries to ensure consumer expectations and regulatory practices. Trends Food Sci Technol. 2016; 52: 1-15. https://doi.org/10.1016/ j.tifs.2016.03.009.
  7. Pöhnl H. Applications of different curing approaches and natural colorants in meat products. in: handbook on natural pigments in food and beverages: industrial applications for improving food color. 1st ed. Woodhead Publishing Series in Food Science, Technology and Nutrition; 2016. p. 209-225. 
  8. Iammarino M, Mentana A, Centonze D, Palermo C, Mangiacotti M, Chiaravalle AE. Dye use in fresh meat preparations and meat products: a survey by a validated method based on HPLC-UV-diode array detection as a contribution to risk assessment. Int J Food Sci Technol. 2020; 55(3): 1126-1135. https://doi.org/10.1111/ijfs.14275.
  9. Müller-Maatsch J, Gras C. The “Carmine Problem” and Potential Alternatives. In: Carl R, Schweiggert RM, editors. Handbook on Natural Pigments in Food and Beverages: Industrial Applications for Improving Food Color. Elsevier; 2016. p. 385-428. 
  10. Lima E, Bosch P, Loera S, Ibarra IA, Laguna H, Lara V. Non-toxic hybrid pigments: Sequestering betanidin chromophores on inorganic matrices. Appl Clay Sci. 2009; 42(3-4): 478-482. https://doi.org/10.1016/j.clay. 2008.06.005.
  11. Zhao X, Zhang X, Tie S, Hou S, Wang H, Song Y, et al. Facile synthesis of nano-nanocarriers from chitosan and pectin with improved stability and biocompatibility for anthocyanins delivery: An in vitro and in vivo study. Food Hydrocoll. 2020; 109: 106114. https://doi.org/10.1016/j.foodhyd.2020. 106114.
  12. Liu J, Zhuang Y, Hu Y, Xue S, Li H, Chen L, et al. Improving the color stability and antioxidation activity of blueberry anthocyanins by enzymatic acylation with p-coumaric acid and caffeic acid. Lwt. 2020; 130: 109673. https://doi.org/10.1016/j.lwt.2020. 109673.
  13. Antigo JLD, Stafussa AP, Bergamasco R de C, Madrona GS. Chia seed mucilage as a potential encapsulating agent of a natural food dye. J Food Eng. 2020; 285: 110101. https://doi.org/10.1016/j.jfoodeng. 2020.110101.
  14. Ongaratto GC, Oro G, Kalschne DL, Trindade Cursino AC, Canan C. Cochineal carmine adsorbed on layered zinc hydroxide salt applied on mortadella to improve color stability. Curr Res Food Sci. 2021; 4: 758-64. https://doi.org/10.1016/j.crfs.2021.10.006
  15. Marangoni R, Ramos LP, Wypych F. New multifunctional materials obtained by the intercalation of anionic dyes into layered zinc hydroxide nitrate followed by dispersion into poly(vinyl alcohol) (PVA). J Colloid Interface Sci. 2009; 330(2): 303-309. https://doi.org/10.1016/j.jcis.2008.10.081.
  16. Zimmermann A, Jaerger S, Zawadzki SF, Wypych F. Synthetic zinc layered hydroxide salts intercalated with anionic azo dyes as fillers into high-density polyethylene composites: First insights. J Polym Res. 2013; 20(224): 1-11. https://doi.org/10.1007/s10965-013-0224-3.
  17. Forano C. Environmental Remediation Involving Layered Double Hydroxides. In: Wypych F, Satyanarayana KG, editors. Clay Surfaces - Fundamentals and Applications. 2004. p. 425-58. 
  18. Fernandes FM, Baradari H, Sanchez C. Integrative strategies to hybrid lamellar compounds: An integration challenge. Appl Clay Sci. 2014; 100: 2-21. https://doi.org/10.1016/j.clay.2014.05.013.
  19. Hwang SH, Han YS, Choy JH. Intercalation of functional organic molecules with pharmaceutical, cosmeceutical and nutraceutical functions into layered double hydroxides and zinc basic salts. Bull Korean Chem Soc. 2001; 22(9): 1019-22. https://doi.org/10. 5012/bkcs.2001.22.9.1019.
  20. Cursino ACT, Zanotelli NC, Giona RM, de Oliveira Basso RL, Mezalira DZ. Multifunctional food supplements based on layered zinc hydroxide salts intercalated with vitamin anions and adsolubilized with vanillin. J Food Sci Technol. 2021; 58: 3963-3971.https://doi.org/10.1007/s13197-020-04859-8
  21. Velázquez-Carriles CA, Carbajal-Arizaga GG, Silva-Jara JM, Reyes-Becerril MC, Aguilar-Uscanga BR, Macías-Rodríguez ME. Chemical and biological protection of food grade nisin through their partial intercalation in laminar hydroxide salts. J Food Sci Technol. 2020; 57: 3252-3258. https://doi.org/10. 1007/ s13197-020-04356-y.
  22. Alnoman M, Udompijitkul P, Paredes-Sabja D, Sarker MR. The inhibitory effects of sorbate and benzoate against Clostridium perfringens type A isolates. Food Microbiol . 2015; 48: 89-98. http://dx.doi.org/10.1016 /j. fm.2014.12.007.
  23. Bloot APM, Kalschne DL, Nogues DRN, Amaral JS, Flores ELM, Colla E, et al. Phytic Acid against Clostridium perfringens Type A: A Food Matrix Study. Foods. 2022; 11(3): 406. https://doi.org/ 10.3390/foods11030406. 
  24. Ruiz-Capillas C, Herrero AM, Tahmouzi S, Razavi SH, Triki M, Rodríguez-Salas L, et al. Properties of reformulated hot dog sausage without added nitrites during chilled storage. Food Sci Technol Int. 2016; 22(1): 21-30. https://doi.org/10.1177/ 1082013214562919. 
  25. Guimarães AS, Guimarães JS, Rodrigues LM, Fontes PR, Ramos A de LS, Ramos EM. Assessment of Japanese radish derivatives as nitrite substitute on the physicochemical properties, sensorial profile, and consumer acceptability of restructured cooked hams. Meat Sci. 2022; 192: 108897. https://doi.org/10. 1016/j.meatsci.2022.108897. 
  26. Myers K, Cannon J, Montoya D, Dickson J, Lonergan S, Sebranek J. Effects of high hydrostatic pressure and varying concentrations of sodium nitrite from traditional and vegetable-based sources on the growth of Listeria monocytogenes on ready-to-eat (RTE) sliced ham. Meat Sci. 2013; 94(1): 69-76. https://doi.org/10.1016/j.meatsci.2012.12.019.
  27. Sindelar JJ, Cordray JC, Olson DG, Sebranek JG, Love JA. Investigating quality attributes and consumer acceptance of uncured, no-nitrate/nitrite-added commercial hams, bacons, and frankfurters. J Food Sci. 2007; 72(8): S551-9. https://doi.org/10.1111/j. 1750-3841.2007.00486.x.
  28. Jo K, Lee S, Jo C, Jeon HJ, Choe JH, Choi YS, et al. Utility of winter mushroom treated by atmospheric non-thermal plasma as an alternative for synthetic nitrite and phosphate in ground ham. Meat Sci. 2020; 166: 108151. https://doi.org/10.1016/j.meatsci. 2020.108151.
  29. Brazil. Ministério da Agricultura, Pecuária e Abastecimento. Instrução Normativa Nº 20, de 31 de julho de 2000. Regulamento técnico de identidade e qualidade de presunto cozido; 2000 p. 1-22. Available from:https://pesquisa.in.gov.br/imprensa/jsp/ visualiza /index.jsp?data=03/08/2000&jornal=1&pagina=55&totalArquivos=88.
  30. Brazil. Agência Nacional de Vigilância Sanitária. Resolução da Diretoria Colegiada-RDC n° 272 Brazil: Estabelece os aditivos alimentares autorizados para uso em carnes e produtos cárneos; 2019 p. 194. Available from: https://pesquisa.in.gov.br/imprensa/jsp/ visualiza/ index.jsp?data=18/03/2019&jornal=515&pagina=194. 
  31. Silva MLN, Marangoni R, Da Silva AH, Wypych F, Schreiner WH. Poly(vinyl alcohol) composites containing layered hydroxide salts, intercalated with anionic azo dyes (tropaeolin 0 and tropaeolin 0). Polimeros. 2013; 23(2): 248-56. https://dx. doi.org/10.4322/S0104-14282013005000026.
  32. Marchesi CM, Cichoski AJ, Zanoelo EF, Dariva C. Influência das condições de armazenamento sobre os pigmentos cárneos e a cor do salame italiano fatiado. Ciência e Tecnol Aliment. 2006; 26(3): 697-704. https://www.scielo.br/j/cta/a/wnD9ScNb3FVpd8VqSBZwm6j/?format=pdf&lang=pt.
  33. Salueña BH, Gamasa CS, Rubial JMD, Odriozola CA. CIELAB color paths during meat shelf life. Meat Sci. 2019; 157: 107889. https://doi.org/10.1016/ j.meatsci. 2019.107889.
  34. Boyles C, Sobeck SJS. Photostability of organic red food dyes. Food Chem. 2020; 315: 126249. https://doi.org/10.1016/j.foodchem.2020.126249
  35. Delgado-Pando G, Allen P, Kerry JP, O’Sullivan MG, Hamill RM. Optimising the acceptability of reduced-salt ham with flavourings using a mixture design. Meat Sci. 2019; 156: 1-10. https://doi.org/10.1016/j. meatsci. 2019.05.010.
Progress in Color, Colorants and Coatings
Volume 17, Issue 4 - Serial Number 55
October 2024
Pages 407-416

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