Institute for Color Science and Technology (ICST) Progress in Color, Colorants and Coatings 2008-2134 16 3 2023 07 01 Fluorescence Quenching Study of Nano Graphene Oxide Interaction with Naphthalimide Dye: Thermodynamic and Binding Characteristics 243 253 81897 10.30509/pccc.2023.167088.1199 EN M. Mahdiani Department of Polymer Engineering, Kish International Campus, University of Tehran, P.O. Box: 141763-3644, Kish Island, Iran Sh. Rouhani Department of Organic Colorant, Institute for Color Science and Technology, P.O. Box: 16765-654, Tehran, Iran Center of Excellence for Color Science and Technology, Institute for Color Science and Technology, P.O. Box: 16765-654, Tehran, Iran P. Zahedi Department of Polymer Engineering, Kish International Campus, University of Tehran, P.O. Box: 141763-3644, Kish Island, Iran School of Chemical Engineering, College of Engineering, University of Tehran, P.O. Box: 11155-4563, Tehran. Iran Journal Article 2023 01 17 <em>A quenching study was reported for interacting a fluorescence naphthalimide derivative with graphene oxide (GO) as a quencher. 1,8-naphthalimide fluorophore with two amine functional side arms (NN2) was synthesized and characterized. Many different 1,8-naphthalimide fluorescence dye derivatives have been designed for fluorescence probe application. Fluorescence quenching-based platforms in nanoscale have extensively been used in sensing systems. Raman, FTIR, UV-Vis, and fluorescence spectroscopic techniques were used to study GO and NN2 characteristics and their photophysical and quenching mechanisms at different temperatures. The results indicated that graphene plays an effective quencher against the naphthalimide molecule, with quenching efficiently at 91 %. The Stern-Volmer analysis results show a mix of static and dynamic quenching mechanisms. The binding constant of the quencher and fluorophore and the number of binding sites have been reported. Thermodynamic parameters of their interaction were evaluated. The negative values of the ΔG confirm that the complexation process is spontaneous. Meanwhile, the positive entropy value confirms the favourable pathway process. </em> https://pccc.icrc.ac.ir/article_81897_3970c96b018df36b943de44b5371ad65.pdf